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The 1313bp luciferase gene sequence in Diaphanes javanus

The 1313bp luciferase gene sequence in Diaphanes javanus


ABSTRACT
Fireflies use a characteristic communication using light in their mating process. The light is produced by peroxisomes in phagocytes which contain the luciferase enzyme. The luciferase enzyme is regulated by the luciferase gene (LUC). The luciferase gene is an important element that is currently being developed as a bioindicator and biosensor. This study aimed to obtain the gene sequence for luciferase and discover the percentage of similarity between the luciferase gene from Diaphanes javanus Gorham and gene bank reference fireflies. The study employed a descriptive analysis method. The data collected were in the form of single electrogram bands, chromatogram sequences and percentages of similarity.
The results of the isolation, restriction, and amplification of the luciferase gene produced a single band and nucleotide readings which were at a length of 1313pb. The luciferase contig sequence for Diaphanes javanus was compared with the gene bank reference sequence. The highest similarity of the luciferase gene in Diaphanes javanus was shown diaphane pectineal at 98% with the number of genes successfully isolated 67% of the total luciferase gene. The lowest similarity rate was 64% when compared to Photuris pennsylvanica. The similarity rate can help determine the direction of the development of the Diaphanes javanus Gorham luciferase gene in Indonesia.

Keywords: Diaphanes javanus, Gorham, Firefly, Luciferase gene, Percentage of similarity


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Detail Information

Bagian Informasi
Pernyataan Tanggungjawab Ria Amelia S.Si., M.Imun.
Pengarang R. Puspitaningrum - Personal Name (Pengarang)
A. Izzatii - Personal Name (Pengarang)
A. Supiyani - Personal Name (Pengarang)
R. Amelia - Personal Name (Pengarang)
Edisi Publish
No. Panggil PUB 42 2017
Subyek
Klasifikasi PUB 42 2017
Judul Seri
GMD Publikasi Dosen
Bahasa
Penerbit
Tahun Terbit 2017
Tempat Terbit Bekasi
Deskripsi Fisik
Info Detil Spesifik ISSN 0971–765X

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Citation

R. Puspitaningrum. (2017).The 1313bp luciferase gene sequence in Diaphanes javanus(Publish).Bekasi:

R. Puspitaningrum.The 1313bp luciferase gene sequence in Diaphanes javanus(Publish).Bekasi:,2017.Publikasi Dosen

R. Puspitaningrum.The 1313bp luciferase gene sequence in Diaphanes javanus(Publish).Bekasi:,2017.Publikasi Dosen

R. Puspitaningrum.The 1313bp luciferase gene sequence in Diaphanes javanus(Publish).Bekasi:,2017.Publikasi Dosen

 



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