Ria Amelia S.Si., M.Imun. Pengarang

Abstract– Lead (Pb) is a toxic pollutant and no organism can decrease the natural concentration. In Kalideres, there is a bus station and industries that are adjacent to settlements, exposing children around to Pb. One of the characteristics of Pb particulates that they are found in the bottom layer of air, ± 1 meter above the ground, equivalent to the height of children aged 6to 9 years old (grade 1 to 2 elementary school students) whose height range between 100-140 cm, increasing the risk of the children being exposed to Pb. The aim of this study was to identify the ALAD gene polymorphism, to discover the frequency of the ALAD gene polymorphism genotype, and to discover the relationship between the ALAD gene polymorphism genotype and the hemoglobin concentration. The method used in this study was a comparative-descriptive method with laboratory test techniques using a digital hemoglobinometer, PCR and PCR-ARMS (Amplification Refractory Mutation System), and the completion of questionnaires by the parents for respondent behavioral characteristics and routines. The samples were 60 students were 51.7% were ALAD wildtypes, 48.3% heterozygote genotypes, and mutants were found in the population of elementary schoolchildren in Kalideres, Jakarta, and there was no connection between the ALAD gene polymorphism genotype and the hemoglobin concentration category (anemic and non-anemic). The results of this research also predict the profile level of the lead environment present as well. Keywords: Lead (Pb), Levulinate dehydratase amino acid, buccal mucosa cells, PCR-ARMS, Anemia.