Siti Nurfajriah S,Pd., M.Si. Pengarang Noor Andryan Ilsan, Ph.D Pengarang

The exploration of alternative natural products is one of the important focuses for a researcher. Microbes are still the best source for searching the bioactive compounds. Actinomycetes are Gram-Positive bacteria which have been known able to produce various secondary metabolite compounds such as antimicrobials and enzyme inhibitors. The aims of this study were to isolate actinomycetes from Moringa oleifera phylloplane also evaluate the antimicrobial and alpha-glucosidase inhibitor activities. Methods of this study including isolated phylloplane actinomycetes from Moringa oleifera leaves, characterized of the actinomycetes colony either morphological or genomic approach, and alpha-glucosidase inhibitory assay. The result of molecular 16s rRNA gene identification showed FKU2 isolate had closest related to Nocardia rhamnosiphila. FKU2 isolate had antimicrobial activities against E. coli, Staphylococcus aureus, Klebsiella pneumonia, K. pneumonia ESBL, and Vibrio sp. in vitro. FKU2 also had alpha-glucosidase inhibitor activity of 7.50 ± 1.59% at 0.01 µg mL-1 extract concentrations compared to acarbose (control) of 8.41 ± 0.43%. Keyword: Actinomycetes, Nocardia sp., alpha-glucosidase inhibitor, antimicrobial, Moringa oleifera