Detail Cantuman

ABSTRACT
Extraintestinal pathogenic Escherichia coli (ExPEC) is a group of pathogens
that can colonize the outside of the intestine, such as the kidney, urinary tract,
or bloodstream. This study analyzed more about the genetic background of
β-lactamase genes among Indonesian ExPEC ST131. Whole-genome sequencing
(WGS) sequences from the National Center for Biotechnology Information
(NCBI) of Indonesian ExPEC ST131 were taken, then analyzed. Circular genomic
mapping and genomic comparison of surrounding genes of β-lactamase in these
isolates were generated. blaOXA-1 and blaDHA-1 were analyzed deeply due to their
presence in a relatively long contig, making them available for analysis. Indonesian
ExPEC ST131 isolates had blaOXA-1 with an identical genetic background of E. coli
originating from China and Austria with aac (6’)-1b-cr5 in the downstream and
cab83 in the upstream. The blaOXA-1 was also detected in other species, including
Klebsiella pneumoniae INF142 originating from Australia and Salmonella enterica
S146 from China. While, blaDHA-1 in EC_0406 had an identical genetic background
to E. coli 142 and other species such as Shigella sonei FC1428 from India and S.
flexneri M2901 from Australia, with Globulin-encoded gene in the upstream and
lysR in the downstream. Our findings demonstrate the global spread of β-lactamase
genes detected in Indonesian ExPEC ST131. These genes were identical to those
isolated from some countries around the world.

KEYWORDS :
β-lactamase, genetic background, extraintestinal pathogenic Escherichia coli, ST131, whole-genome sequencing